Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216 |
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Authors: | Song Hui-Ting Jiang Zheng-Bing Ma Li-Xin |
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Institution: | College of Chemistry and Material Science, Hubei University, Wuhan, PR China. |
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Abstract: | We isolated two lipase genes LIPY7, LIPY8 from Yarrowia lipolytica CGMCC (China general microbiological culture collection center) AS 2.1216. The LIPY7 and LIPY8 genes encode a 366 and a 371-amino acid protein, respectively. The lipase genes with 6 x His tag sequence were cloned into expression vector pPIC9K and successfully integrated into a heterologous fungal host Pichia pastoris KM71, respectively. The recombinants were induced by methanol to secrete active lipases into cultural medium. The recombinant lipases were also purified and characterized. |
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Keywords: | Cloning Expression Lipase Purification |
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