Rapid immuno‐monitoring of inoculant plant growth‐promoting microorganisms |
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| Authors: | Mihály L Kecskés Michael T Rose Erwin Michel Benôit Lauby Malala Rakotondrainibe Andrea Casteriano Attila Palágyi Anaëlle Moutouvirin Sébastien Elter Romain Guillas Ganisan Krishnen Ivan R Kennedy |
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| Institution: | 1. SUNFix Centre for Nitrogen Fixation, Faculty of Agriculture, Food and Natural Resources, The University of Sydney, New South Wales, Australia;2. The University of Brest, ESMISAB, Brest, France;3. Szent István University, G?d?ll?, Hungary |
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| Abstract: | Rapid, specific techniques are essential to monitor the quality of inoculant plant growth‐promoting strains at all stages of manufacture from starter culture to the final product in its carrier medium. In this study, colony immunoblotting was evaluated for the specific detection and enumeration of Citrobacter freundii, one component of a Vietnamese commercial inoculant plant growth‐promoting product used to improve the yield and nutrient efficiency of paddy rice. For quality control of either sterilised or unsterilised carrier media in commercial products colony immunoblotting proved to be a promising tool. Furthermore, it was possible using this technique to measure the survival of this strain in soil and the rhizosphere. |
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| Keywords: | Plant growth‐promoting bacteria Colony immunoblotting ELISA |
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