Pulsed electromagnetic field enhances brain-derived neurotrophic factor expression through L-type voltage-gated calcium channel- and Erk-dependent signaling pathways in neonatal rat dorsal root ganglion neurons |
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| Institution: | 1. Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran;2. Department of Biotechnology, Iranian Research Organization for Science and Technology, Tehran, Iran;3. Department of Genetics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran;1. Department of Medicine and Aging Science, University ‘G. d''Annunzio’ of Chieti-Pescara, Via dei Vestini, Chieti, Italy;2. King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia;3. Department of Bioengineering, Integral University, Kursi Road, Lucknow, UP, India;4. Department of Experimental and Clinical Sciences, University \"G.d''Annunzio\" Chieti-Pescara, Chieti, Italy;1. 3B''s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal;2. ICVS/3B''s – PT Government Associate Laboratory, Braga, Guimarães, Portugal |
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| Abstract: | Although pulsed electromagnetic field (PEMF) exposure has been reported to promote neuronal differentiation, the mechanism is still unclear. Here, we aimed to examine the effects of PEMF exposure on brain-derived neurotrophic factor (Bdnf) mRNA expression and the correlation between the intracellular free calcium concentration (Ca2+]i) and Bdnf mRNA expression in cultured dorsal root ganglion neurons (DRGNs). Exposure to 50 Hz and 1 mT PEMF for 2 h increased the level of Ca2+]i and Bdnf mRNA expression, which was found to be mediated by increased Ca2+]i from Ca2+ influx through L-type voltage-gated calcium channels (VGCCs). However, calcium mobilization was not involved in the increased Ca2+]i and BDNF expression, indicating that calcium influx was one of the key factors responding to PEMF exposure. Moreover, PD098059, an extracellular signal-regulated kinase (Erk) inhibitor, strongly inhibited PEMF-dependant Erk1/2 activation and BDNF expression, indicating that Erk activation is required for PEMF-induced upregulation of BDNF expression. These findings indicated that PEMF exposure increased BDNF expression in DRGNs by activating Ca2+- and Erk-dependent signaling pathways. |
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| Keywords: | Electromagnetic field Calcium Dorsal root ganglion neurons Brain-derived neurotrophic factor Erk inhibitor |
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