Permethrin resistance variation and susceptible reference line isolation in a field population of the mosquito,Culex quinquefasciatus (Diptera: Culicidae) |
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作者姓名: | Ting Yang Nannan Liu |
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作者单位: | Department of Entomology and Plant Pathology, Auburn University, Auburn, AL 36849, USA |
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基金项目: | Acknowledgments The project described was supported by Award Number R21AI076893 to N.L. from the National Institute of Allergy and Infectious Diseases, AAES Hatch/Multistate Grants ALA08-045 and ALA015-1-10026 to N.L. |
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摘 要: | This study examines the genetic variations and mechanisms involved in the development of permethrin resistance in individual mosquitoes from a field population of Culex quinquefasciatus, HAmCq^G0, and characterizes susceptible reference lines of mosquitoes with a similar genetic background to the field HAmCq^G0 strain. Six upregulated cytochrome P450 genes, CYP9M10, CYP9J34, CYP6P14, CYP9J40, CYP6AA7, and CYP4C52v1, previously identified as being upregulated in the larvae of resistant HAmCq68 mosquitoes were examined in the larvae of 3 strains (susceptible S-Lab, parental HAmCq^G0 and permethrin-selected highly resistant HAmCq68) and 8 HAmCq^G0 single- egg raft colonies, covering a range of levels of susceptibility/resistance to perrnethrin and exhibiting different variations in the expression of A and/or T alleles at the L-to-F kdr locus of the sodium channel. The 2 lines with the lowest tolerance to permethrin and bearing solely the susceptible A allele at the L-to-F kdr locus of the sodium channels, from colonies Cx_SERC5 and Cx_SERC8, showed lower or similar levels of all 6 of the P450 genes tested compared with the S-Lab strain, suggesting that these 2 lines could be used as the reference mosquitoes in future studies characterizing insecticide resistance in HAmCq mosquitoes. This study also provides a detailed investigation of the mechanisms involved in insecticide resistance in individuals within a population: individuals with elevated levels of resistance to permethrin all displayed one or more potential resistance mechanisms-either elevated levels of P450 gene expression, or L-to-F mutations in the sodium channel, or both.
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关 键 词: | 电阻变化 致倦库蚊 氯菊酯 参考线 细胞色素P450基因 蚊子 野外种群 基因表达水平 |
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