| 黑翅土白蚁纤维素酶纯化及其特性研究(英文) |
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| 引用本文： | 杨天赐,莫建初,程家安.黑翅土白蚁纤维素酶纯化及其特性研究(英文)[J].Entomologia Sinica,2004(1). |
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| 作者姓名： | 杨天赐 莫建初 程家安 |
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| 作者单位： | 浙江大学城市昆虫学研究中心，浙江大学城市昆虫学研究中心，浙江大学城市昆虫学研究中心 杭州，310029，杭州，310029，杭州，310029 |
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| 摘 要： | 利用DuoFlow层析系统阴离子交换柱UNOsphere Q,从黑翅土白蚁工蚁体内分离出内切葡聚糖酶。以羧甲基纤维素和水杨苷为底物,测得纯化酶的活性分别是原酶液的807.5和14.4倍。经变性聚丙烯酰胺凝胶电泳分析,获得单一的蛋白条带,其分子量大小为80kDa。经双向电泳图谱分析,其等电点为6.4。
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| 关 键 词： | 中纤维素酶 内切葡聚糖酶 黑翅土白蚁 酶纯化 双向电泳 |
PURIFICATION AND SOME PROPERTIES OF CELLULASE FROM ODONTOTERMES FORMOSANUS (ISOPTERA:TERMITIDAE) |
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| Authors: | Tian-ci Yang Jian-chu Mo and Jia-an Cheng Urban Entomology Research Center Zhejiang University Hangzhou Zhejiang P R China |
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| Institution: | Tian-ci Yang,Jian-chu Mo,and Jia-an Cheng Urban Entomology Research Center,Zhejiang University,Hangzhou,Zhejiang 310029,P. R. China |
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| Abstract: | The purification of the cellulase from Odontotermes formosanus workers was achieved by using anion-exchange column of UNOsphere Q, BioLogic DuoFlow chromatogra phy system. The purified cellulase was identified as an endoglucanase and some of its properties were investigated. The EGase activity was 807.5-fold as high as the initial enzyme activity using CMC as substrate and 14.4-fold using salicin as substrate. The enzyme preparations were homogeneous as judged by SDS-PAGE electrophoresis, molecular weight of which was 80 kDa and confirmed by 2-DE zymogram analysis. The enzyme was isoelectric at pH 6.4, which was active on CMC substrate. |
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| Keywords: | cellulase endoglucanase Odontotermes formosanus enzyme purification two dimentional gel electrophoresis |
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