| 磷酸酯酶与乙酰胆碱酯酶在棉铃虫对辛硫磷抗药性中的作用(英文) |
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| 引用本文： | 汤锋,岳永德,花日茂.磷酸酯酶与乙酰胆碱酯酶在棉铃虫对辛硫磷抗药性中的作用(英文)[J].Entomologia Sinica,2001(1). |
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| 作者姓名： | 汤锋 岳永德 花日茂 |
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| 作者单位： | 安徽农业大学植物保护系!合肥230036 |
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| 摘 要： | 利用人工选育的方法 ,获得了棉铃虫对辛硫磷的抗药性品系和相对敏感品系 ,其对辛硫磷的LD50分别为 1 1 .0 574μg/虫和 0 .9790 μg/虫。以生物测定与生化测定的方法 ,比较了两品系的差异。增效剂活体试验测定结果表明 ,磷酸三苯酯、增效磷对棉铃虫三龄幼虫均表现出一定的增效作用 ,但在两品系间的增效作用存在差异 ,对相对敏感品系的增效倍数分别为 2 .2 7倍、2 .1 3倍 ,对抗性品系的增效倍数分别为 6.93倍和 6.43倍。磷酸酯酶活性测定结果表明 ,抗性品系的酶活力分别是相对敏感品系的1 6.67倍 (碱性 )和 1 .89倍 (酸性 ) ,Km 和Vmax则分别为 0 .76倍、2 .64倍 (碱性 )和 1 .38倍、1 .78倍 (酸性 ) ;而羧酸酯酶的活性则差异不大。对靶标酶乙酰胆碱酯酶的活性测定表明 ,与相对敏感品系相比 ,抗性品系酶活性显著增强 ,并且对杀虫剂的亲和力降低 ,说明乙酰胆碱酯酶在棉铃虫对辛硫磷的抗药性中也有着重要的作用
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| 关 键 词： | 棉铃虫 辛硫磷 抗药性机理 磷酸酯酶 乙酰胆碱酯酶 |
THE ROLE OF PHOSPHOTASE AND ACETYLCHOLINESTE RASE IN RESISTANCE TO PHOXIM IN HELICOVERPA ARMIGERA |
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| Authors: | TANG Feng YUE Yong\|de and HUA Ri\|mao |
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| Institution: | TANG Feng,YUE Yong\|de and HUA Ri\|mao Department of Plant Protection,Anhui Agricultural University,Hefei 230036,China |
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| Abstract: | The resistance mechanism underlying phoxim resistance was investigated through biochemical assays. The results show that there are marked relations between the phosphatase activity and resistance to phoxim. Furthermore, there is a significant difference in the K i values of AChE between the two strains and it is inferred that the change in ability of binding to phoxim is a mechanism responsible for resistance to phoxim. The metabolic inhibitors Triphenyl Phosphate (TPP) and O,O\|diethyl\|O\|phenyl\|thiophosphate (SV 1) displayed significant synergism of phoxim toxicity to the two strains. It implied that esterase might be involved in resistance. However, there is no marked correlation between resistance and CarE. |
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| Keywords: | Helicoverpa armigera resistance mechanism acetylcholinesterase esterase phoxim |
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