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RAPD方法用于区分中国烟粉虱的生物型
引用本文:吴杏霞,胡敦孝,等.RAPD方法用于区分中国烟粉虱的生物型[J].Entomologia Sinica,2002,9(3):1-8.
作者姓名:吴杏霞  胡敦孝
作者单位:中国农业大学昆虫学系,北京100094
摘    要:应用RAPD-PCR技术研究了中国大陆10个烟粉虱种群的生物型情况,聚类分析表明,这10个种群中包含了3种生物型:烟粉虱B型,广西型(南宁南瓜寄主)和福建型(福州地瓜)。值得注意的是,在福建并存着两种烟粉虱生物型,即苷蓝上的B型烟粉虱和甘署上的福建福州型,研究表明烟粉虱B型已经传入中国,至少在北京,山东,新疆,福建,广东等地都有发生和分布,广西型和福建型是否中国的本地土种群还需进一步研究。

关 键 词:RAPD方法  区分  中国  烟粉虱  生物型

USING RAPD\|PCR TO DISTINGUISH BIOTYPES OF BEMISIA TABACI (HOMOPTERA:ALEYRODIDAE) IN CHINA
Authors:WU Xing xia  HU Dun xiao\  LI Zheng xi and SHEN Zuo rui
Institution:WU Xing xia,HU Dun xiao\+*,LI Zheng xi and SHEN Zuo ruiDepartment of Entomology,China Agricultural University,Beijing\ 100094
Abstract:Ten populations of the whitefly Bemisia tabaci (Gennadius) collected from eight fieed and ornamental crops from seven provinces/cities in China were determined using RAPD PCR. Results showed that the 10 populations of B.tabaci could be classified into three distinct groups: a Biotype B group, a Guangxi group, and a Fujian group. The whiteflies in the Biotype B group were collected from sweetpotato and cotton in Beijing, tomato in Shandong, poinsettia in Shanghai and Guangdong, cabbage in Fujian, and hemp and eggplant in Xinjiang. The Guangxi group were collected from pumpkin in Nanning, Guangxi, and the Fujian group, from sweetpotato in Fujian. It was notable that B.tabaci specimens collected on cabbage in Fujian belonged to the Biotype B group. It is clear that Biotype B does now occur in China, in Beijing, Shandong, Xinjiang, Shanghai, Fujian and Guangdong. It is likely that the Guangxi and Fujian groups are native to China.
Keywords:Bemisia tabaci    B Biotype  RAPD  PCR  
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