| 长角血蜱幼虫中的蜕皮甾类(英文) |
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| 引用本文： | 刘敬泽,赵秀颖.长角血蜱幼虫中的蜕皮甾类(英文)[J].Entomologia Sinica,1999(3). |
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| 作者姓名： | 刘敬泽 赵秀颖 |
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| 作者单位： | 河北师范大学生物系!石家庄050016(刘敬泽)，河北医科大学图书馆!石家庄050017(赵秀颖) |
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| 基金项目： | the Youth Natural Science Foundation of Hebei Normal University of China |
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| 摘 要： | 本文用放射免疫分析和高效液相色谱法对长角血蜱幼虫中的蜕皮甾类进行了测定。 蜕皮甾类存在于吸血和饱血幼虫，并且随生理时期而变化。吸血期和饱血后前３天，激素含量低（小于 １４． ３５ｐｇ ＥＥ／只）；饱血后５天迅速上升（２９．８７ｐｇ ＥＥ／只）；饱血后７天达到高峰（５６． ０４ｐｇ ＥＥ／只）；高峰后又下降到低水平（１９．３８ ｐｇＥＥ／只）。高峰期的蜕皮甾类主要为两种成分，２０Ｅ和Ｅ（２０Ｅ∶Ｅ＝３．０７∶１）。２０Ｅ可能是幼虫发育中起主要作用的激素。
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ECDYSTEROIDS IN THE LARVA OF HAEMAPHYSALIS LONGICORNIS NEUMANN (ACARI: IXODIDAE) |
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| Authors: | Jingze Liu and Xiuying Zhao |
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| Abstract: | The ecdysteroids (ECs) concentration and their components in the larvae of Haemaphysalis longicornis were determined by radioimmunoassay (RIA) and high performance liquid chromatography (HPLC). ECs ware found in feeding and engorged larvae and absent in unfed larvae. During feeding and the first 3 days after engorgement, ECs were in low level (less than 14. 35pg EE /larva). On the sib day, they began to increase sharply (29.87pg EE/larva) and reached their peak (56. 04pg EE/larva) on the 7th day. Then they declined to low value again (19. 38pg EE/larva). HPLC analysis revealed that the ECs components of the RIA peak could be 20-hydroxyecdysone (20E) and ecdysone (E) on the ratio of 20E: E=3.07: 1, which meant 20E was probably the main effective hormone. |
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| Keywords: | Haemaphysalis longicornis larva ecdysteroids |
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