| 中华蜜蜂蜂毒磷脂酶A_2基因在大肠杆菌中的表达(英文) |
| |
| 引用本文： | 沈立荣,程家安,张传溪.中华蜜蜂蜂毒磷脂酶A_2基因在大肠杆菌中的表达(英文)[J].Entomologia Sinica,2004(1). |
| |
| 作者姓名： | 沈立荣 程家安 张传溪 |
| |
| 作者单位： | 浙江大学应用昆虫学研究所，浙江大学应用昆虫学研究所，浙江大学应用昆虫学研究所 杭州 310029，杭州 310029，杭州 310029 |
| |
| 摘 要： | 将中华蜜蜂蜂毒磷脂酶A2(AcPLA2)蛋白成熟肽编码区基因(405 bp)克隆至表达载体pETBlue-1,在大肠杆菌Tuner(DE3)plac I中诱导表达,经SDS-PAGE电泳检测,表达产物分子量为15kD,约占细菌总蛋白的4.6％;用意大利蜜蜂蜂毒磷脂酶A2(AmPLA2)纯品制备的兔源多克隆抗体为一抗作Western blot,表达产物显示类似于天然纯AmPLA2的特异性印迹,证实AcPLA2基因已在大肠杆菌中得到表达。
|
| 关 键 词： | 中华蜜蜂 蜂毒磷脂酶A2 表达 大肠杆菌 |
EXPRESSION OF A BEE-VENOM PHOSPHOLIPASE A_2 FROM APIS CERANA CERANA IN ESCHERICHIA COLI |
| |
| Authors: | Li-rong Shen Jia-an Cheng and Chuan-xi Zhang
Institute of Applied Entomology Zhejiang University Huajiachi Campus Hangzhou China |
| |
| Institution: | Li-rong Shen,Jia-an Cheng and Chuan-xi Zhang
Institute of Applied Entomology,Zhejiang University,Huajiachi Campus,Hangzhou 310029,China |
| |
| Abstract: | The venomous phospholipase A2 (AcPLA2) coding reading region of the Chinese honeybee (Apis cerana cerana), which is composed of 405 bp encoding a mature glycosylated peptide with 134 amino residues was transformed into the expression vector pETblue-1. Then the recombinant vector was introduced into Escherichia coli Tuner (DE3) plac I for expression. Analysis result of SDS-PAGE showed that the expression products had a protein band of about 15kD. Detection of western blot using ant-European honeybee (Apis mellifera) phospholipase A2 (AmPLA2) polyclonal serum as the first antibody showed that the expression products appeared a special blot same as the native AmPLA2.The result demonstrated that the AcPLA2 peptide had been expressed in E. coli and the AcPLA2 has the similar antigenicity as the AmPLA2. |
| |
| Keywords: | Apis cerana cerana venomous phospholipase A2 E coli expression |
| 本文献已被 CNKI 等数据库收录！ |
