Molecular characterization of mesophilic and thermophilic sulfate reducing microbial communities in expanded granular sludge bed (EGSB) reactors |
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Authors: | Stephanie A Freeman Reyes Sierra-Alvarez Mahmut Altinbas Jeremy Hollingsworth Alfons J M Stams Hauke Smidt |
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Institution: | (1) Department of Chemical and Environmental Engineering, University of Arizona, P.O. Box 21001, Tucson, AZ 85721, USA;(2) Laboratory of Microbiology, Wageningen University, P.O. Box 8033, 6700 EJ Wageningen, The Netherlands;(3) Department of Environmental Engineering, Istanbul Technical University, Istanbul, Turkey |
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Abstract: | The microbial communities established in mesophilic and thermophilic expanded granular sludge bed reactors operated with sulfate
as the electron acceptor were analyzed using 16S rRNA targeted molecular methods, including denaturing gradient gel electrophoresis,
cloning, and phylogenetic analysis. Bacterial and archaeal communities were examined over 450 days of operation treating ethanol
(thermophilic reactor) or ethanol and later a simulated semiconductor manufacturing wastewater containing citrate, isopropanol,
and polyethylene glycol 300 (mesophilic reactor), with and without the addition of copper(II). Analysis, of PCR-amplified
16S rRNA gene fragments using denaturing gradient gel electrophoresis revealed a defined shift in microbial diversity in both
reactors following a change in substrate composition (mesophilic reactor) and in temperature of operation from 30°C to 55°C
(thermophilic reactor). The addition of copper(II) to the influent of both reactors did not noticeably affect the composition
of the bacterial or archaeal communities, which is in agreement with the very low soluble copper concentrations (3–310 μg l−1) present in the reactor contents as a consequence of extensive precipitation of copper with biogenic sulfides. Furthermore,
clone library analysis confirmed the phylogenetic diversity of sulfate-reducing consortia in mesophilic and thermophilic sulfidogenic
reactors operated with simple substrates. |
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Keywords: | Anaerobic wastewater treatment Copper Ethanol Methanogens Sulfate reducing bacteria DGGE 16S rRNA gene clone library |
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