Plant gene responses to frequency-specific sound signals |
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Authors: | Mi-Jeong Jeong Chang-Ki Shim Jin-Ohk Lee Hawk-Bin Kwon Yang-Han Kim Seong-Kon Lee Myeong-Ok Byun Soo-Chul Park |
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Institution: | (1) National Institute of Agricultural Biotechnology, Rural Development Administration (RDA), Suwon, 441-707, Korea;(2) National Institute of Agricultural Science and Technology, Rural Development Administration, Suwon, 441-707, Korea;(3) Division of Applied Biological Sciences, Sunmoon University, Asan, 336-708, Korea;(4) Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, Taejeon, 305-701, Korea |
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Abstract: | We identified a set of sound-responsive genes in plants using a sound-treated subtractive library and demonstrated sound regulation
through mRNA expression analyses. Under both light and dark conditions, sound up-regulated expression of rbcS and ald. These are also light-responsive genes and these results suggest that sound could represent an alternative to light as a
gene regulator. Ald mRNA expression increased significantly with treatment at 125 and 250 Hz, whereas levels decreased significantly with treatment
at 50 Hz, indicating a frequency-specific response. To investigate whether the ald promoter responds to sound, we generated transgenic rice plants harboring a chimeric gene comprising a fusion of the ald promoter and GUS reporter. In three independent transgenic lines treated with 50 or 250 Hz for 4 h, GUS mRNA expression was up-regulated at 250 Hz, but down-regulated at 50 Hz. Thus, the sound-responsive mRNA expression pattern
observed for the ald promoter correlated closely with that of ald, suggesting that the 1,506 bp ald promoter is sound-responsive. Therefore, we propose that in transgenic plants, specific frequencies of sound treatment could
be used to regulate the expression of any gene fused to the ald promoter. |
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Keywords: | Aldolase Frequency-specific expression Rice Sound-induced genes |
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