Using microsatellites,isozymes and AFLPs to evaluate genetic diversity and redundancy in the cassava core collection and to assess the usefulness of DNA-based markers to maintain germplasm collections |
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Authors: | Chavarriaga-Aguirre Paul Maya María M Tohme Joe Duque Myriam C Iglesias Carlos Bonierbale Merideth W Kresovich Steve Kochert Gary |
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Institution: | (1) Biotechnology Research Unit, The University of Georgia, currently at, Colombia and;(2) Cassava Program, CIAT, Apartado Aéreo 6713, Cali, Colombia;(3) Formerly at CIAT, currently at, International Potato Center (CIP), Apartado 1558, Lima 12, Peru;(4) USDA-ARS/Plant Genetic Resources Conservation Unit, University of Georgia, Griffin, GA 30223-1797, USA;(5) 2502 Plant Sciences, Department of Botany, University of Georgia, Athens, GA 30605, USA |
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Abstract: | The cassava core collection was selected to represent, with minimum repetitiveness, the potential genetic diversity of the
crop. The core (630 accessions) was chosen from the base collection (over 5500 accessions) on the basis of diversity of origin
(country and geographic), morphology, isozyme patterns and specific agronomic criteria. To asses the genetic diversity of
the core, 521 accessions were typed with four microsatellite loci. Allele diversity and frequency, and size variance of dinucleotide
repeats (Rst statistic) were estimated. Microsatellite allele numbers and frequencies varied among countries: Colombia and
Brazil had the largest number of different alleles across all loci. Mexico also had a high number, ranking fifth after Peru,
Costa Rica and Venezuela (which tied). Unique alleles were present in accessions from Brazil, Colombia, Guatemala, Venezuela
and Paraguay. A small number (1.34%) of potential duplicates were identified through isozyme and AFLP profiles. Thus, the
present results indicated that traditional markers have been highly effective at selecting unique genotypes for the core.
Future selections of cassava germplasm sets can be aided by DNA-based markers to ensure genetically representative, non-redundant
samples.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | Manihot esculenta Crantz core collection molecular markers germplasm characterization and diversity |
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