Constitutive expression of a cowpea trypsin inhibitor gene,CpTi, in transgenic rice plants confers resistance to two major rice insect pests |
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| Authors: | Deping Xu Qingzhong Xue David McElroy Yogesh Mawal Vaughan A Hilder Ray Wu |
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| Institution: | (1) Field of Plant Biology and Section of Biochemistry, Molecular and Cell Biology, Cornell University, 14853 Ithaca, NY, USA;(2) Present address: Research and Product Development, Research Center, Pioneer Hi-Bred International, Inc., 50131 Johnston, IA, USA;(3) Department of Agronomy, Zhejiang Agricultural University, 310029 Hangzhou, P. R. China;(4) Present address: USDA/ARS Plant Gene Expression Center, 800 Buchanan Street, 94710 Albany, CA, USA;(5) Present address: Centre de Recherche, Hopital Sainte-Justine, 3175 Cote Sainte-Catherine, H3T 1C5 Montreal, Quebec, Canada;(6) Axis Genetics Ltd., Babraham, CB2 4ZA Cambridge, UK |
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| Abstract: | The gene encoding a cowpea trypsin inhibitor (CpTI), which confers insect resistance in trangenic tobacco, was introduced into rice. Expression of the CpTi gene driven by the constitutively active promoter of the rice actin 1 gene (Act1) leads to high-level accumulation of the CpTI protein in transgenic rice plants. Protein extracts from transgenic rice plants exhibit a strong inhibitory activity against bovine trypsin, suggesting that the proteinase inhibitor produced in transgenic rice is functionally active. Small-scale field tests showed that the transgenic rice plants expressing the CpTi gene had significantly increased resistance to two species of rice stem borers, which are major rice insect pests. Our results suggest that the cowpea trypsin inhibitor may be useful for the control of rice insect pests. |
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| Keywords: | cowpea trypsin inhibitor insect resistance proteinase inhibitor stem borer transgenic rice |
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