High-level Expression of Maize <Emphasis Type="Italic">γ</Emphasis>-zein Protein in Transgenic Soybean (<Emphasis Type="Italic">Glycine max</Emphasis>) |
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Authors: | Zhiwu?Li Sarah?Meyer Juliane S?Essig Ying?Liu Melissa A?Schapaugh S?Muthukrishnan Bryan E?Hainline Email author" target="_blank">Harold N?TrickEmail author |
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Institution: | (1) Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA;(2) Department of Statistics, Kansas State University, Manhattan, KS 66506, USA;(3) Department of Biochemistry, Kansas State University, Manhattan, KS 66506, USA;(4) Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN 46202-5225, USA |
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Abstract: | Primers were developed for 118 microsatellites isolated from grape (Vitis vinifera) genomic libraries enriched for (AC)n repeats. Only one microsatellite sequence matched other grape SSR-sequences in the GeneBank database. Genotyping was carried out in the parental lines and four offspring of two pseudo-test-cross populations, Cabernet Sauvignon x Seyval and Chardonnay x Bianca, and a further six other grape genotypes (V. vinifera Sultanina, Merlot, Syrah, Müller-Thurgau, Vitis Regent and V. riparia Gloire de Montpellier). A total of 108 microsatellites showed easily scorable alleles and 100 of them segregated according to a configuration suitable for mapping in either cross. A further 8 SSRs, although unsuitable for mapping in those crosses, showed polymorphism in the other genotypes tested. This set of markers was used, along with 75 microsatellites of other repeat-types, to fingerprint 46 offspring of the cross Chardonnay x Bianca. For each full-sib, individual heterozygosity and distance in repeat units between pairs of alleles at each locus (mean d2) were calculated as a tool for predicting highly outbred recombinant individuals. Six microsatellites with segregation ratios significantly distorted towards the lack of homozygous sibs were identified and mapped to linkage groups LG 3 and LG 5. Estimation of heterozygosity at genome-wide level and genotyping at loci for which homozygous sibs are discriminated against are discussed for marker-assisted background selection in outcrossing grapevines. |
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Keywords: | Genetic transformation Nutritional value Seed storage proteins Sulfur-containing amino acid |
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