A mutation at the Ala122 position of acetohydroxyacid synthase (AHAS) located on chromosome 6D of wheat: improved resistance to imidazolinone and a faster assay for marker assisted selection |
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Authors: | D Li I Barclay K Jose K Stefanova R Appels |
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Institution: | (1) Molecular Plant Breeding CRC, State Agriculture Biotechnology Centre, Murdoch University, South Street, Perth, WA, 6150, Australia;(2) Department of Agriculture and Food Western Australia, Baron-Hay Court, South Perth, WA, 6151, Australia;(3) Centre for Comparative Genomics, Murdoch University, South Street, Perth, WA, 6150, Australia |
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Abstract: | A new mutation at the acetohydroxyacid synthase (AHAS) locus on chromosome 6D of wheat was analyzed in detail because it conferred
an improved resistance to the imidazolinone group of herbicides. Sequence analysis showed that the mutation was at the Ala122
position (A122T), a position in AHAS which has not to date been identified in imidazolinone resistant wheat lines even though
the position has been identified in other plants and is associated with resistance. An allele-specific assay for the mutation
(in the wheat line Brookton-8) was developed and used in a genetic analysis. Two mapping populations were analysed and the
doubled haploid progeny from the cross Brookton-8 × Clearfield STL proved to be most informative. The AHASAla122 mutation (A122T) was allelic to the AHASSer653 mutation (S653N) in Clearfield STL (Imi1, on chromosome 6D) and hence was assigned to the chromosome 6D locus. The analysis
of the doubled haploid lines in the mapping population demonstrated the greater resistance conferred by the A122T mutation
because lines from the same cross and carrying either the A122T or S653N mutations could be directly compared.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Wheat Imidazolinone resistance AHAS mutations Single step PCR assay |
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