Effects of combined expression of antifungal barley seed proteins in transgenic wheat on powdery mildew infection |
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Authors: | Bieri Stéphane Potrykus Ingo Fütterer Johannes |
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Institution: | (1) Institute of Plant Sciences, ETH Zürich, Universitätstrasse 2, Zürich, CH-8092, Switzerland |
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Abstract: | Transgenicwheat plants (variety Frisal) constitutively expressing a number of potentialantifungal proteins alone or in combinations were generated and tested forincreased resistance to Blumeria graminis f.sp. tritici(powdery mildew) in a detached leaf infection assay. The most significativerateof protection was obtained with an apoplastic ribosome-inactivation proteinfrombarley seed. Apoplastic Barnase was less efficient and individual plant linesharbouring a barley seed chitinase and -1,3-glucanase showed linespecificphenotypes from increased resistance to increased susceptibility. Combinationbycrossing of three barley seed proteins did not lead to significant improvementof protection. |
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Keywords: | -1" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-1 3-glucanase Anti-fungal protein Chitinase Fungal tolerance Ribosome inactivating protein Synergistic effect Transgenic wheat |
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