Cloning of <Emphasis Type="Italic">TaTPP-6AL1</Emphasis> associated with grain weight in bread wheat and development of functional marker |
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Authors: | Pengfei Zhang Zhonghu He Xiuling Tian Fengmei Gao Dengan Xu Jindong Liu Weie Wen Luping Fu Genying Li Xinxia Sui Xianchun Xia Chunping Wang Shuanghe Cao |
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Institution: | 1.College of Agronomy,Henan University of Science and Technology,Luoyang,China;2.Institute of Crop Science, National Wheat Improvement Center,Chinese Academy of Agricultural Sciences,Beijing,China;3.International Maize and Wheat Improvement Center (CIMMYT) China Office,Chinese Academy of Agricultural Sciences,Beijing,China;4.Keshan Sub-Academy,Heilongjiang Academy of Agricultural Sciences,Keshan,China;5.Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan,China |
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Abstract: | Trehalose 6-phosphate phosphatase (TPP) dephosphorylates trehalose 6-phosphate to trehalose, an important growth regulator, and is involved in starch accumulation and grain yield. In this study, wheat TPP homologs were isolated from chromosomes 6AL, 6BL, and 6DL, designated as TaTPP-6AL1, TaTPP-6BL1, and TaTPP-6DL1, respectively. Sequence alignment showed a single-nucleotide polymorphism (SNP) at TaTPP-6AL1 locus between cultivars with contrasting thousand grain weight (TGW), forming alleles TaTPP-6AL1a and TaTPP-6AL1b, respectively. A cleaved amplified polymorphic sequence (CAPS) marker, TaTPP-6AL1-CAPS, was developed to differentiate the two alleles. TaTPP-6AL1 was mapped within the interval of IWB65749 and IWB60449 in a recombinant inbred line (RIL) population derived from Zhou8425B/Chinese Spring using the wheat 90K SNP assay. A QTL for TGW identified in the interval explained 12.1–19.1% of the phenotypic variance across five environments. Association analysis on 141 Chinese wheat cultivars also indicated a significant correlation of TaTPP-6AL1 with TGW. In conclusion, TaTPP-6AL1 and its functional marker are valuable to improve grain yield in wheat breeding. |
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