Identification and functional analysis of the Pm4CL1 gene in transgenic tobacco plant as the basis for regulating lignin biosynthesis in forest trees |
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| Authors: | Ha Van Huan Ho Van Giang Ngo Van Thanh Siliang Zhang Yong Wang |
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| Institution: | (1) Department of Biotechnology, Viet Nam Forestry University, Xuan Mai, Ha Noi, Vietnam;(2) State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237 Shanghai, People’s Republic of China;(3) Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200032 Shanghai, People’s Republic of China |
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| Abstract: | Reducing the lignin content of trees could provide both economic and environmental benefits. To this end, the coumarate:coenzyme
A ligase 1 gene (4CL1) was isolated from Pinus massoniana Lamb (Pm4CL1). The sequence of the full-length Pm4CL1 cDNA (accession no. FJ810495) contained an entire open reading frame (ORF) of 1,614 bp, which encoded a polypeptide of 537
amino acid residues. Tobacco (Nicotiana tabacum L.) as a model plant was used for functional characterization of the Pm4CL1 gene in transgenic plants. Results revealed that 4CL1 enzyme activity and lignin content in most antisense Pm4CL1 transgenic tobacco lines were decreased as compared to wild-type; the average 4CL1 enzyme activity was decreased by 48.75%
and lignin content was decreased by 24.5%. In contrast, in the sense Pm4CL1 transgenic tobacco lines, average 4CL1 enzyme activity was increased by 72.3% and lignin content was increased by 27.6%.
These results suggest that the Pm4CL1 gene from P. massoniana could be applied to regulate lignin biosynthesis in transgenic trees. |
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