Genetic variation through Dissociation (Ds) insertional mutagenesis system for rice in Korea: progress and current status |
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Authors: | Dong-Soo Park Soo-Kwon Park Sang-Ik Han Hoe-Jeong Wang Nam-Soo Jun Norvie L Manigbas Young-Min Woo Byoung-Ohg Ahn Doh-Won Yun Ung-Han Yoon Yong-Hwan Kim Myung-Chul Lee Doh-Hoon Kim Min-Hee Nam Chang-Deok Han Hang-Won Kang Gihwan Yi |
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Institution: | (1) Functional Crop Resource Development Division, Department of Functional Crop, NICS, RDA, 1085 Neidong, Milyang, Gyeong-nam, 627-803, Korea;(2) Pohang University of Science and Technology, Pohang, Korea;(3) Dong-A University, Busan, Korea;(4) Gyeongsang National University, Jinju, Korea;(5) National Academy of Agricultural Science, RDA, Suwon, 441-707, Korea |
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Abstract: | A gene detection strategy using two-component Ac/Ds construct, with the mobile Ds transposon, has been developed to better understand gene functions in crops. Currently, 115,000 Ds insertion lines have been generated through the Ac/Ds gene trap system in Korea using japonica rice Dongjin as donor. Four hundred and thirty-seven mutants from 12,162 Ds-tagged lines were catalogued, including physiological and agronomic traits. Different traits were identified with distinct
characteristics in terms of tillers, panicles, leaves, flowers, seed, chlorophyll content, and height. Culm and panicle length,
number of panicles, and days to flowering of the Dongjin Ds population revealed high standard deviations compared with the donor cultivar. An evaluation of the Ds distribution on the chromosome revealed that 74.5% of the Ds were reinserted into gene-rich regions, making this Ac/Ds-mediated gene trap system useful in helping to gain an understanding of the function of genes and thus improve the gene-tagging
system in rice. |
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Keywords: | Ac/Ds insertion mutagenesis Genetic variation Morphological traits Rice |
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