Formation in vitro of ripe tomato fruits from thin layer explants of flower pedicels |
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Authors: | Ravindar Kaur-Sawhney Philip B Applewhite Arthur W Galston |
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Institution: | (1) Department of Biology, Yale University, 06511 New Haven, Connecticut, USA |
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Abstract: | Thin longitudinal sections cut from pedicels of fifteen cultivars of tomato (Lycopersicon esculentum) were grown in vitro on Murashige-Skoog medium supplemented with various concentrations of different auxins and cytokinins. Isatin (an auxin precursor slowly converted to an active auxin) was the most effective source of auxin for the formation of buds without prior root formation, while zeatin was the most effective cytokinin for growth and development of the buds. Flower buds and ripe fruits developed consistently from explants of the cultivar Pixie Hybrid II treated with 10 M isatin plus 3 M zeatin as the cytokinin. Fruits developed parthenocarpically, grew to a diameter of about 15 mm, ripened promptly, and possessed normal color and flavor.Abbreviations BAP
benzylaminopurine
- IAA
indole-3-acetic acid
- IBA
indole-3-butyric acid
- IPA
isopentyladenosine
- NAA
-napthaleneacetic acid |
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Keywords: | flower buds Lycopersicon esculentum in vitro pedicel explants ripe fruits |
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