Protective roles of nitric oxide on germination and antioxidant metabolism in wheat seeds under copper stress |
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Authors: | Kang-Di Hu Lan-Ying Hu Yan-Hong Li Fen-Qin Zhang Hua Zhang |
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Institution: | (1) School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui, 230009, People’s Republic of China;(2) Department of Biology, Hexi University, Zhangye, Gansu, 734000, People’s Republic of China |
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Abstract: | Nitric oxide (NO) is a multifunctional gaseous signal in plant. In the present study, we found that pretreatment with NO could
significantly improve wheat seeds germination and alleviate oxidative stress against copper toxicity. With the enhancement
of copper stress, the germination percentage of wheat seeds decreased gradually. Pretreatment during wheat seed imbibition
with sodium nitroprusside (SNP), an NO donor, could greatly reverse the inhibitory effect of the following copper stress to
wheat seeds germination. SNP-pretreated seeds also tended to retain higher amylase activities than that of the control without
SNP pretreatment. On the other hand, there was no apparent difference in the activities of esterase in wheat seeds pretreated
with or without SNP. Further investigations showed that pretreatment with NO donor dramatically stimulated the activities
of superoxide dismutase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6), decreased the activities of lipoxygenases, sustained
a lower level of malondialdehyde, and interfered with hydrogen peroxide (H2O2) excessive accumulation compared with the control, thereby enhancing the antioxidative capacity in wheat seeds under copper
stress. In addition, the seed copper contents were not significant different between those pretreated with SNP and the controls,
inferring that protective roles of NO was not responsible for preventing Cu uptake.
Kang-Di Hu and Lan-Ying Hu contributed equally to this paper. |
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Keywords: | Antioxidative enzymes Copper stress Nitric oxide Seeds germination Wheat (Triticum) |
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