Improvement of cold tolerance of the half-high bush Northland blueberry by transformation with the <Emphasis Type="Italic">LEA</Emphasis> gene from <Emphasis Type="Italic">Tamarix androssowii</Emphasis> |
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Authors: | Xin Zhao Li-Ping Zhan Xue-Zhong Zou |
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Institution: | (1) College of Forestry, Northeast Forestry University/Key Laboratory of Forestry Genetics and Breeding, 150040 Harbin, Heilongjiang Province, China;(2) Liaoning Forestry Vocation-Technical College, 110101 Shenyang, Liaoning Province, China;(3) The Professional and Technological Department of Liaoning University of TCM, 110101 Shenyang, Liaoning Province, China |
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Abstract: | Previous studies have shown that the late embryogenesis abundant (LEA) gene of Tamarix androssowii can enhance the drought tolerance of transgenic tobacco. In this study, the cloned LEA gene was transformed into half-high
bush Northland blueberry in order to enhance its ability to tolerate cold stress. The cephalosporin antibiotics ceftriaxone,
cefotaxime and cefazolin were used to optimize transformation of leaf explants, and kanamycin sulfate was used to select for
transgenic shoots. PCR and Southern blot analysis confirmed 8 transformants with LEA gene copy numbers ranging from 1 to 7.
The LEA chimeric gene was found to be normally transcribed in 6 transgenic lines by RT-PCR. The 8 transgenic lines were tested
for cold tolerance by measuring the activities of peroxidase (POD) and superoxide dismutase (SOD), malondialdehyde (MDA) content
and relative electrolyte leakage (REL). Overexpression of the LEA gene enhanced the activity of both POD and SOD under low
temperature stress conditions. Lipid peroxidation in the transgenic lines was significantly lower than in non-transgenic plants
after cold stress, as determined by MDA content and REL. Thus, our findings indicate that the LEA gene confers increased cold
tolerance in the Northland blueberry and implicate the metabolic pathways through which it exerts this effect. |
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