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Vitrification of carnation in vitro: Changes in cell wall mechanical properties,cellulose and lignin content |
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| Authors: | C Kevers R Prat Th Gaspar |
| Institution: | (1) Hormonologie fondamentale et appliquée, Institut de Botanique B22, Université de Liège, Sart Tilman, B-4000 Liège, Belgium;(2) Laboratoire des Biomembranes et Surfaces cellulaires végétales, Ecole Normale supérieure, 24, rue Lhomond, F-75231 Paris, France;(3) Institut de Botanique, Université de Liège, B 22, Sart Tilman, B-4000 Liège, Belgium |
| Abstract: | Vitrification of internodes of carnation was brought about by culturing in liquid medium. Cell wall extensibility of these internodes was kinetically followed in comparison to that of normal plants using the constant stress method. Liquid culture induced increased immediate and total deformation capacities of the walls from the second day. Measurements indicated that these deformation capacities involved plastic properties rather than elastic ones. These changes were paralleled by decreased relative levels of cellulose and lignin. |
| Keywords: | vitrification Dianthus caryophyllus cell wall extension cellulose lignification carnation |
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