Intravital microscopy technique to study parasite dynamics in the labyrinth layer of the mouse placenta |
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| Institution: | 1. CEDOC, Departamento de Imunologia, Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisboa, Portugal;2. Escuela Superior de Ingeniería Informatica, University of Vigo, Vigo 32004, Spain;3. Departmento of Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil;4. Instituto Gulbenkian de Ciência, Oeiras, Portugal;1. Unité de Biologie et Genétique du Paludisme, Institut Pasteur, Paris, France;2. Parasite Disease Group, IBMC—Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal;1. CEA, Division of Immno-Virology, iMETI, Fontenay-aux-Roses Cedex, France;2. UMR E1, Université Paris Sud 11, Orsay, France;3. Center for Infectious Disease Models and Innovative Therapies (IDMIT), Fontenay-aux-Roses Cedex, France;4. CNRS, UMR3569, Paris, France;1. Barcelona Centre for International Health Research (CRESIB, Hospital Clínic-Universitat de Barcelona) ISGlobal, Barcelona, Spain;2. Institute of Cell Biology, University of Bern, Bern, Switzerland;3. Leiden Malaria Research Group Parasitology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands;4. Advanced Light Microscopy Unit, School of Medicine, Scientific and Technological Centers, University of Barcelona, Spain;5. Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain |
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| Abstract: | Intravital imaging techniques are the best approach to investigate in situ cellular behavior under physiological conditions. Many techniques have emerged during these last few years for this purpose. We recently described an intravital imaging technique that allows for the observation of placenta physiological responses at the labyrinth layer of this tissue. This technique will be very useful to study many placental opportunistic infections and in this article we reinforce its usefulness by analyzing placental physiological entrapment of beads and parasites. In particular, our results show that small beads (1.0 μm) or Plasmodium chabaudi-GFP-infected-Red Blood Cells (Pc-GFP-iRBCs) cannot get trapped inside small or large blood vessels of popliteal lymph nodes (PLNs). Inside the placenta, clusters of beads could only be found inside the maternal blood vessels. However, Pc-GFP-iRBCs were found inside and outside the maternal blood vessels. We observed that trophoblasts can ingest infected-Red Blood Cells (iRBCs) in vitro and immunofluorescence of placenta revealed Pc-GFP-iRBCs inside and outside the maternal blood vessels. Taken together, we conclude that fast deposition of particles inside blood vessels seems to be an intrinsic characteristic of placenta blood flow, but iRBCs could be internalized by trophoblast cells. Thus these results represent one of the many possible uses of our intravital imaging technique to address important questions inside the parasitological field. |
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