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A scanning electron microscope study of Craspedella sp. from the branchial chamber of redclaw crayfish,Cherax quadricarinatus,from Queensland,Australia
Authors:Kim B Sewell  Lester R G Cannon
Institution:(1) The Queensland Museum, Cultural Centre, PO Box 3300, 4101 South Brisbane, Queensland, Australia;(2) The Department of Parasitology, The University of Queensland, 4072 Brisbane, Queensland, Australia;(3) The Department of Anatomical Sciences, The University of Queensland, 4072 Brisbane, Queensland, Australia
Abstract:Epidermal topography was examined, including papillate ridges, grooves and ciliated sensory papillae of Craspedella sp. from the branchial chamber of redclaw crayfish, Cherax quadricarinatus, from Queensland, Australia. Rhandites were observed to discharge from ducts opening mainly in a small distal region of the ventral epidermis of the three central (of five) tentacles. These regions, devoid of ciliated sensory papillae, serve to adhere the anterior end of the worms during locomotion. Secretions from glands associated with the posterior attachment organ were observed to discharge from pores on the outside region of the ventral surface of the disc.A comparison of various scanning electron microscopy (SEM) fixation techniques showed that (1) hot fixatives at 90 °C provide most information on the largest number of epidermal structures and (2) different fixation regimes highlight different epidermal features.
Keywords:Temnocephalidae  Craspedella  ectosymbiotic flatworms  scanning electron microscopy  epidermis  methods of fixation
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