Molecular cut-off values of dialysis membranes for alginate and kappa-carrageenan oligosaccharides |
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| Authors: | Knutsen Svein H Moe Størker T Larsen Bjørn Grasdalen Hans |
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| Institution: | (1) Division of Biotechnology, The Norwegian Institute of Technology, University of Trondheim, N-7034 NTH Trondheim, Norway |
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| Abstract: | The effective molecular weight cut-off values of dialysis membranes for carrageenan and alginate oligosaccharides were evaluated by gel permeation chromatography and nuclear magnetic resonance spectroscopy. For the different membranes tested, i.e. Medicell, Spectra Por 1000D and 3500D, the porous sizes are analogous to tri- and tetrasaccharides. A simple dialysis can be used to recover the majority of the oligosaccharides produced by a carrageenase or an alginate lyase digestion. |
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| Keywords: | Kappa-carrageenan alginate oligosaccharides fractionation dialysis size |
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