Cytogenetic studies in South American species of Serjania (Sapindaceae: Paullinieae) |
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Authors: | J D Urdampilleta M S Ferrucci A L L Vanzela |
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Institution: | 1. Instituto Multidisciplinario de Biología Vegetal (IMBIV), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)?–?Universidad Nacional de Córdoba (UNC) , Córdoba , Argentina juanurdampilleta@hotmail.com;3. Instituto de Botánica del Nordeste (IBONE), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)?–?Universidad Nacional del Nordeste (UNNE) , Corrientes , Argentina;4. Laboratório de Biodiversidade e Restaura??o de Ecossistemas, Departamento de Biología Geral , Centro de Ciências Biológicas, Universidade Estadual de Londrina, Campus Universitário , Londrina , Paraná , Brazil |
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Abstract: | Abstract Serjania Mill. (Paullinieae) is considered the most important neotropical genus of Sapindaceae due to species number and its widespread distribution. In this study, 14 species belonging to three sections were analyzed using conventional staining, C/CMA/DAPI banding, and fluorescence in situ hybridization (FISH) with a 18S-5.8S-26S rDNA probe. New chromosome counts are reported for Serjania crassifolia, Serjania platycarpa, and Serjania regnellii, all with 2n = 24, which is remarkably constant for Serjania. The karyotypes are moderately asymmetric, and variations observed in A1 and A2 indices show resemblances between S. platycarpa, Serjania hebecarpa, and S. crassifolia, and between Serjania communis, Serjania gracilis, and S. regnellii. The banding pattern was homogeneous in Serjania. C/DAPI bands (AT-rich sites) were not clearly evidenced, but changes in the number and position of GC-rich sites (CMA bands) were observed. These segments were associated with 18S-5.8S-26S rDNA sites. The significance of the results is discussed in relation to chromosomal data available for the genus and in regard to the infrageneric treatment of Serjania. |
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Keywords: | 18S-5 8S-26S rDNA Giemsa C-banding fluorochrome banding FISH cytotaxonomy karyotype symmetry Serjania |
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