Production of FMDV virus-like particles by a SUMO fusion protein approach in Escherichia coli |
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Authors: | Chien-Der Lee Yao-Pei Yan Shu-Mei Liang Ting-Fang Wang |
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Institution: | (1) Institute of Molecular Biology, Academia Sinica, Taipei, 11529 Taiwan, Republic of China;(2) Agriculture Biotechnology Research Center, Academia Sinica, Taipei, 11529 Taiwan, Republic of China |
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Abstract: | Virus-like particles (VLPs) are formed by the self-assembly of envelope and/or capsid proteins from many viruses. Some VLPs
have been proven successful as vaccines, and others have recently found applications as carriers for foreign antigens or as
scaffolds in nanoparticle biotechnology. However, production of VLP was usually impeded due to low water-solubility of recombinant
virus capsid proteins. Previous studies revealed that virus capsid and envelope proteins were often posttranslationally modified
by SUMO in vivo, leading into a hypothesis that SUMO modification might be a common mechanism for virus proteins to retain water-solubility
or prevent improper self-aggregation before virus assembly. We then propose a simple approach to produce VLPs of viruses,
e.g., foot-and-mouth disease virus (FMDV). An improved SUMO fusion protein system we developed recently was applied to the
simultaneous expression of three capsid proteins of FMDV in E. coli. The three SUMO fusion proteins formed a stable heterotrimeric complex. Proteolytic removal of SUMO moieties from the ternary
complexes resulted in VLPs with size and shape resembling the authentic FMDV. The method described here can also apply to
produce capsid/envelope protein complexes or VLPs of other disease-causing viruses. |
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