The N-terminal domain of desmin is not involved in intermediate filament formation: evidence from thrombic digestion studies |
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Authors: | Yi-juan Lu Peter Johnson |
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Institution: | Department of Chemistry and College of Osteopathic Medicine, Ohio University, Athens, Ohio 45701, USA |
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Abstract: | Thrombic digestion of chicken gizzard desmin resulted in the cleavage of six arginyl bonds in the desmin molecule (at arginine residues 27, 33, 48, 59, 67 and 96), all of which are located in the N-terminal globular domain of the molecule. The large thrombic fragment (residues 97–463) of desmin isolated from this digest, which contains the central rod and C-terminal non-helical regions of desmin, was found to form filamentous structures indistinguishable from those of intact desmin. These results indicate that, in contrast to previous predictions, the N-terminal domain of desmin is not essential for intermediate filament formation. |
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Keywords: | Protein desmin intermediate filamets thrombin muscle |
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