Multiple effects of differentiation-inducing factor on prespore differentiation and cyclic-AMP signal transduction in Dictyostelium |
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| Authors: | Mei Wang Peter JM Van Haastert Panline Scbaap |
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| Institution: | Cell Biology and Morphogenesis Unit, Zoological Laboratory, University of Leiden, Kaiserstraat 63, NL-2311 GP Leiden, The Netherlands |
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| Abstract: | Abstract. The effects of the differentiation inducing factor (DIF) on several CAMP-induced responses in Dictyostelium were investigated. It was found that DIF reduces the apparent affinity of cell-surface cAMP receptors. DIF does not affect the CAMP-induced cGMP response, but it is a potent inhibitor of the CAMP-relay response. DIF also inhibits the induction of prespore differentiation by cAMP in aggregation-competent cells. We also compared the effects of DIF on CAMP-induced responses with those of the relay inhibitor, caffeine, and the morphogen, adenosine. |
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