Osmotic Adjustment of Cultured Tobacco Cells (Nicotiana tabacum var. Samsum) Grown on Sodium Chloride

Tobacco cell cultures (var. Samsum) were grown on increasing levels of NaCl to select variants for increased salt tolerance. The osmotic adjustment of NaCl-adapted and nonadapted cell lines was studied. Both cell lines were grown on modified Linsmaier and Skoog medium with or without NaCl. Few differences were found in the response of adapted and nonadapted lines to NaCl.