ClC-2 Activation Modulates Regulatory Volume Decrease |
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| Authors: | H Xiong C Li E Garami Y Wang M Ramjeesingh K Galley CE Bear |
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| Institution: | (1) Division of Cell Biology, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada M5G 1X8, CA;(2) Department of Physiology, University of Toronto, 1 King's College Circle, Toronto, Canada, CA |
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| Abstract: | ClC-2 belongs to a large family of chloride channels and its expression in certain cell types is associated with the appearance
of swelling-activated chloride (Cl−) currents. In the present report, we examined the hypothesis that ClC-2 plays a role in regulatory volume decrease by expressing
ClC-2 in Sf9 cells using the baculovirus system. First, we showed that ClC-2 protein expression is associated with appearance
of a Cl− conductance which is activated by hypo-osmotic shock and can be distinguished from swelling-activated chloride currents endogenous
to Sf9 cells on the basis of its pharmacology and specific inhibition by an anti-ClC-2 antibody. Second, we show that the
rate of regulatory volume decrease is significantly enhanced in Sf9 cells expressing ClC-2 protein. Hence, our data support
the hypothesis that ClC-2 is capable of mediating regulatory volume decrease.
Received: 12 August/Revised: 23 October 1998 |
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| Keywords: | : ClC-2 — Chloride channels — Patch-clamp electrophysiology — Baculovirus expression — Anti-ClC-2 antibody — Regulatory volume decrease |
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