Electrorotation of Erythrocytes Treated with Dipicrylamine: Mobile Charges within the Membrane Show their ``Signature' in Rotational Spectra |
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Authors: | VL Sukhorukov U Zimmermann |
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Institution: | Lehrstuhl für Biotechnologie der Universit?t Würzburg, Am Hubland, D-97074 Würzburg, Germany, DE
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Abstract: | In this study, electrorotation spectra of individual cells (that is, frequency dependence of cell rotation speed) have been
proved to yield information not only about the passive electric properties of cell constituents, but also about the presence
of mobile charges within the plasma membrane being part of ion carrier transport systems. Experiments on human erythrocytes
pretreated with the lipophilic anion dipicrylamine (DPA) gave convincing evidence that these artificial mobile charges adsorbed
to the plasma membrane contributed significantly to the rotational spectrum at relatively low conductivity of the external
medium (2–5 mS m−1). Theoretical integration of the mobile charge concept into the single-shell model (viewing the cell as a homogenous sphere
surrounded by a membrane) led to a set of equations which predicted electrorotational behavior of DPA-treated cells in dependence
on medium conductivity. The quantitative data on the partition and the transmembrane translocation rate of the DPA anion extracted
from the experimental rotational spectra agreed well with the corresponding literature values.
Received: 14 February 1996/Revised: 29 May 1996 |
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Keywords: | : Mobile charges — Dielectric Spectroscopy — Electrorotation — Dispersion — Membrane Capacity — Membrane Conductivity — Lipophilic Ion |
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