|
|||||
|
|
Phosphoinositide 3-Kinase Pathway Mediates Early Aldosterone Action on Morphology and Epithelial Sodium Channel in Mammalian Renal Epithelia |
|
| Authors: | Yuan Zhou Xuewei Chen Xiao Liu Hujie Lu Ying Li Hui Zhu Gaihong An Na Zhang Jianning Zhang Qiang Ma Yanjun Zhang |
| Institution: | 1. Department of Neurosurgery, Tianjin Medical University General Hospital; Tianjin Neurological Institute; Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education; Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, 154 Anshan Road, Heping District, Tianjin, 300052, China 2. Department of Occupational Hygiene, Institute of Health and Environmental Medicine, 1 Dali Road, Heping District, Tianjin, 300050, China 3. Nanomedicine Laboratory, China National Academy of Nanotechnology & Engineering, TEDA, Tianjin, 300457, China 4. Medicine Division, Imperial College London, London, W12 0NN, UK |
| Abstract: | Involvement of phosphoinositide 3-kinases (PI3Ks) in early aldosterone action on epithelial sodium channel (ENaC) in mammalian renal epithelia was investigated by hopping probe ion conductance microscopy combined with patch-clamping in this study. Aldosterone treatment enlarged the cell volume and elevated the apical membrane of renal mpkCCDc14 epithelia, which resulted in enhancing the open probability of ENaC. Inhibition of PI3K pathway by LY294002 obviously suppressed these aldosterone-induced changes in both cell morphology and ENaC activity. These results indicated the important role of PI3K pathway in early aldosterone action and the close relationship between cell morphology and ENaC activity in mammalian renal epithelia. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |