Increasing cell biomass in <Emphasis Type="Italic">Saccharomyces cerevisiae</Emphasis> increases recombinant protein yield: the use of a respiratory strain as a microbial cell factory |
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| Authors: | Cecilia Ferndahl Nicklas Bonander Christel Logez Renaud Wagner Lena Gustafsson Christer Larsson Kristina Hedfalk Richard AJ Darby Roslyn M Bill |
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| Institution: | 1.Chemical and Biological Engineering/Molecular Biotechnology,Chalmers University of Technology,G?teborg,Sweden;2.School of Life and Health Sciences,Aston University,Birmingham,UK;3.UMR 7175 - LC1, Dpt Récepteurs et Protéines Membranaires,ESBS,Illkirch Cedex,France;4.Department of Chemistry/Biochemistry,G?teborg University,G?teborg,Sweden |
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| Abstract: | Background Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given
protein required for applications as diverse as structural genomics and biopharmaceutical manufacture. Yeast is a well-established
recombinant host cell for these purposes. In this study we wanted to investigate whether our respiratory Saccharomyces cerevisiae strain, TM6*, could be used to enhance the productivity of recombinant proteins over that obtained from corresponding wild
type, respiro-fermentative strains when cultured under the same laboratory conditions. |
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