Quantification of the physiochemical constraints on the export of spider silk proteins by <Emphasis Type="Italic">Salmonella</Emphasis> type III secretion |
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Authors: | Daniel M Widmaier Christopher A Voigt |
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Institution: | 1.Chemistry and Chemical Biology Graduate Program,University of California - San Francisco,San Francisco,USA;2.Department of Pharmaceutical Chemistry,University of California - San Francisco,San Francisco,USA |
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Abstract: | Background The type III secretion system (T3SS) is a molecular machine in gram negative bacteria that exports proteins through both membranes
to the extracellular environment. It has been previously demonstrated that the T3SS encoded in Salmonella Pathogenicity Island 1 (SPI-1) can be harnessed to export recombinant proteins. Here, we demonstrate the secretion of a variety
of unfolded spider silk proteins and use these data to quantify the constraints of this system with respect to the export
of recombinant protein. |
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