| 应用荧光原位杂交和染色体配对研究八倍体小冰麦中2的染色体组构成及染色体特征 |
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| 引用本文： | 高智,韩方普,何孟元,马有志,辛志勇.应用荧光原位杂交和染色体配对研究八倍体小冰麦中2的染色体组构成及染色体特征[J].Acta Botanica Sinica,1999(1). |
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| 作者姓名： | 高智 韩方普 何孟元 马有志 辛志勇 |
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| 作者单位： | 东北师范高等专科学校!四川乐山６１４００４(高智，韩方普)，东北师范大学遗传与细胞研究所!长春１３００２４(何孟元)，中国农业科学院作物育种栽培研究所!北京１０００８１(马有志，辛志勇) |
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| 摘 要： | 通过染色体配对分析和荧光原位杂交（ＦＩＳＨ）技术对八倍体小冰麦中２的染色体组构成进行分析，结果表明：八倍体小冰麦中２含有的冰草染色体是来自天蓝冰草（Ａｇｒｏｐｙｒｏｎ ｉｎｔｅｒｍｅｄｉｕｍ（Ｈｏｓｔ）Ｐ．Ｂ．＝Ｅｌｙｔｒｉｇｉａ ｉｎｔｅｒｍｅｄｉａ（Ｈｏｓｔ）Ｎｅｖｓｋｉ＝Ｔｈｉｎｏｐｙｒｕｍ ｉｎｔｅｒｍｅｄｉｕｍ （Ｈｏｓｔ）Ｂａｒｋｗｏｒｔｈ ａｎｄ Ｄｅｗｅｙ）具同亲关系的染色体组，但冰草的这种同亲关系的染色体组不同于二倍体长穗偃麦草（Ｔｈｉｎｏｐｙｒｕｍ ｅｌｏｕｇａｔｕｍ ２Ｘ）的Ｅ组染色体。中２含有１２条冰草染色体，且有一对染色体为小麦（Ｔｒｉｔｉｃｕｍ ａｅｓｔｉｖｕｍ Ｌ．）染色体和冰草染色体之间易位所形成的。
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| 关 键 词： | 小麦 天蓝冰草 八倍体小冰麦 染色体配对 荧光原位杂交 |
Characterization of Genome and Chromosomes in Octoploid Wheat-wheatgrass Amphiploid Zhong 2 Using Fluorescence in situ Hybridization and Chromosome Pairing Analysis |
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| Authors: | GAO Zhi HAN Fang-Pu HE Meng-Yuan |
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| Abstract: | Genomic constitution of octoploid wheat-wheatgrass amphiploid Zhong 2 was analyzed by chromo- some pairing and fluorescence in situ hybridization techniques. The results indicated that the octoploid wheatwheatgrass chromosomes in Zhong 2 were derived from the distant homologous genomes of wheatgrass (Agropy-ron intermedium (Host) P. B. = Elytrigia intermedia (Host) Nevski = Thinopyrum intermeedium (Host) Bark-worth and Dewey, and these distant homologous genomes were not from the E genome of T. elongatum 2x.Zhong 2 contained 12 wheatgrass chromosomes in which a pair of chrosomes was involved in translocation between wheatgrass and wheat chromosomes. |
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| Keywords: | Common wheat Wheatgrass Octoploid wheat-wheatgrass Chromosome pairing Fluorescence in situ hybridization |
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