固氮酶结构基因在联合固氮菌Klebsiellaplanticola19—1细胞中的定位 |
| |
引用本文: | 李久蒂,靳红帆.固氮酶结构基因在联合固氮菌Klebsiellaplanticola19—1细胞中的定位[J].Acta Botanica Sinica,1994,36(10):755-758. |
| |
作者姓名: | 李久蒂 靳红帆 |
| |
作者单位: | 中国科学院植物研究所!北京100044 |
| |
摘 要: | 用Klebsiella pneum oniaenifHDK 为探针与Klebsiella planticola 19-1 DNA Southern 杂交表明,K.planticola 19-1 中存在与nifHDK 同源的片段。用凝胶内溶菌及电场倒转技术检测在K.planticola 19-1 中存在一大质粒。用K.pneum oniae nif HDK 探针与大质粒DNASouthern 杂交证明固氮酶结构基因定位于大质粒
|
关 键 词: | Klebsiellaplanticola 固氮酶结构基因 大质粒 基因定位 |
LOCALIZATION OF NITROGEN FIXATION GENE IN AN ASSOCIATED NITROGEN FIXING BACTERIA KLEBSIELLA PLANTICOLA 19 1 |
| |
Authors: | Li Jiu di and Jin Hong fan |
| |
Abstract: | A large plasmid in Klebsiella planticola 19 1 was identified from lysis of bacteria in an agarose gel and using a technique of electrical field overturn.The presence of nif HDK like gene and localization of nif HDK like gene in the large plasmid were confirmed by Southern hybridization with 32 P labelled nif HDK probe. |
| |
Keywords: | Klebsiella planticola nif HDK Large plasmid Localization of gene |
本文献已被 CNKI 维普 等数据库收录! |