野生大豆种子蛋白含量差异的生理及结构基础的探讨

利用ＳＤＳ－聚丙烯酰胺凝胶电泳、电子显微镜、蛋白及酰脲含量测定等技术，对高蛋白含量（５０．７％ ）的５０３５９ 和低蛋白含量（４０．８％ ）的５０３０５ 两个野生大豆在种子发育过程中贮藏蛋白积累的速率、蛋白组分合成的起始时间、蛋白体发育的进程以及幼茎的酰脲含量进行了比较研究。结果表明：野生大豆５０３５９的高蛋白含量是与其种子发育过程中较高的植株酰脲含量、较早较快的贮藏蛋白合成及积累速率，液泡中高效的蛋白贮藏方式以及蛋白体在子叶细胞中占有较大体积相联系的

THE PHYSIOLOGICAL AND STRUCTURAL BASES FOR CONTENT DIFFERENCE IN SEED PROTEIN OF WILD SOYBEAN (GLYCINE SOJA)

The accumulation rate of storage proteins, the intial time of synthesis of protein subunits, the sequence of protein body development and the acyl urea contents in young stem were studied, during seed development in two wild soybean (Glycine soja) plants one with higher protein content (50 7%) and one with lower protein content (40 8%), using the methods of SDS PAGE, electronmicroscopy, and protein and acyl urea and N 2 fixition activity determinations.The results showed that the higher protein content in G.soja was related to the higher N 2 fixation activity, early synthesis and accumulation rates of storage protein, the highly efficent style of protein storage in vacuoles and larger volumes of protein bodies in the cotyledon cells.