| ipt基因定位表达对转基因烟草育性的影响 |
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| 引用本文： | 耿飒,麻密.ipt基因定位表达对转基因烟草育性的影响[J].Acta Botanica Sinica,2000,42(2):217-220. |
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| 作者姓名： | 耿飒 麻密 |
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| 作者单位： | 中国科学院植物研究所,北京 |
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| 基金项目： | 中国科学院院长基金特别支持项目!(960 82 0 0 0 2 1 9)，中国科学院重大项目子课题!(KY951_A1_30 2_1 2_1 2 )，中国科学院生命科学与 |
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| 摘 要： | 杂种优势是生物界的一种普遍现象。作物杂种优势的利用是培育高产、抗逆、优质新品种的重要手段之一。然而 ,常规育种技术获得配套三系的难度很大 ,由此限制了杂种优势利用的发展。近年来应用转基因技术创造雄性不育植株已有不少报道1- 5] 。有研究表明 ,自然突变的雄性不育株
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| 关 键 词： | TA29－ipt基因 育性 转基因烟草 杂种优势 |
Effects on Fertility in Transgenic Tobacco by Localized Expression of ipt Gene |
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| Authors: | GENG Sa MA Mi LI Guo_Feng YE He_Chun [WTBZ] |
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| Institution: | GENG Sa,MA Mi,LI Guo_Feng,YE He_Chun [WT6BZ] |
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| Abstract: | The isopenteryl transferase ( ipt ) gene from Agrobacterium tumefaciens (Smith et Townsend) Conn was driven under the tobacco TA29 promoter and introduced into tobacco (Nicotiana tabacum L.) plants by A. tumefaciens mediated transformation. PCR and Southern blot analysis confirmed that the ipt gene has integrated into the genomes of tobacco plants. The expression pattern of this chimeric TA29_ ipt gene in the transgenic plants was studied, and the endogenous cytokinin level in different organs was assayed by ELISA method. The results showed that the cytokinin content in the androecium of transgenic plants increased 3-4 times as compared with the control, and some changes of the fertility of the TA29_ ipt transgenic plants have been observed. |
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| Keywords: | TA29_ ipt gene fertility transgenic tobacco |
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