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外源Ca^2+对烟草花粉管生长和生殖核分裂的调节
引用本文:范六民,杨弘远.外源Ca^2+对烟草花粉管生长和生殖核分裂的调节[J].Acta Botanica Sinica,1997,39(10):899-904.
作者姓名:范六民  杨弘远
作者单位:武汉大学生命科学学院植物生殖生物学研究室,武汉大学生命科学学院植物生殖生物学研究室,武汉大学生命科学学院植物生殖生物学研究室 武汉 430072,武汉 430072,武汉 430072
摘    要:用细胞学和统计学方法研究了外源Ca~(2 )对烟草(Nicotiana tabacum L.)离体花粉管生长和生殖核分裂的影响。正常培养条件下,花粉管群体内的生殖核分裂率大致呈对数增长,10~18h为其分裂高峰期。所用Ca~(2 )浓度中以10~(-3)mol/L最适于花粉管生长,与之相比,其它浓度随时间延长愈益明显地表现出抑制效应。生殖核分裂则以10~(-2)与10~(-3)mol/L较为适宜,且10~(-2)mol/L可相对提前分裂高峰。在含10~(-3)mol/L Ca~(2 )培养基中培养10h后用不同方法处理,发现高钙抑制花粉管生长,尤以10~(-1)mol/L Ca~(2 )抑制最强烈,导致花粉管顶端壁加厚及生殖核的无丝分裂。而10~(-2)mol/L Ca~(2 )在处理早期(10~12h)促进生殖核分裂。EGTA处理则同时抑制花粉管生长和生殖核分裂。

关 键 词:烟草  花粉管生长  生殖核分裂  外源钙

EXOGENOUS Ca~(2 ) REGULATION OF POLLEN TUBE GROWTH AND DIVISION OF GENERATIVE NUCLEUS IN NICOTIANA TABACUM
Authors:Fan Liu-min  Yang Hong-yuan Zhou Chang
Abstract:Cytological and statistical studies on the effects of exogenous Ca2 on in vitro pollen tube growth and generative nucleus (GN) division of tobacco (Nicotiana tabacum L.) were conducted in an artificial experimental system. Under normal cultured conditions, the rate of GN division increased logarithmically in general,and reaches the climax at about 10 ~ 18 h. Among the treatments with various Ca2 concentrations, 10-3 mol/L was the optimal concentration for pollen tube growth, whereas other Ca2 concentrations showed increasing inhibitory effect with the time of culture. Generally, Ca2 concentrations at 10-2 and 10-3 mol/L favored GN division more than the others. Compared with 10-3 mol/L Ca2 concentration at 10-2 mol/L benefitiated GN division at earlier stage of the treatment, but afterwards showed inhibitory effect gradually. Besides, the authors designed another series of experiments, in which 10-2,10-1 mol/L Ca2 (final concentrations) or 2,10 mmol/L EG-TA was respectively added to the medium containing 10-3 mol/L Ca2 at 10 h of culture. Pollen tube growth was inhibited by the high Ca2 treatments, especially being severely effected by 10-1 mol/L Ca2 from which wall, thickening of the tube tip, amitotic division of GN leading to micronu-cleus formation occurred. 10-2 mol/L Ca2 treatment,however,promoted GN division at the earlier stage of treatment (10~12 h).EGTA treatments inhibited both pollen tube growth and GN division.
Keywords:Nicotiana tabacum  Pollen tube growth  Generative nucleus division  Exogenous calcium ion
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