甜椒花药绒毡层的二型性及其组织化学研究

在甜椒（Capsicum annuum L.)中，靠近花粉中部的绒毡层自药隔产生，由较大的细胞组成，而花药外部区域的其余的绒毡层细胞较小，来自于初生壁层，前者的细胞具有大液泡和较大的细胞核，甲基绿－派罗宁和汞－溴酚蓝染色反应较后者弱，在造孢组织时期，二者液泡内都含有较大的球形的酸性磷酸酶颗粒，在以后的发育中，这种颗粒消失，在减数分裂时期，两种绒毡层的DNA，RNA和蛋白质合成活动增强，来自药隔的绒毡层积累了更多的DNA，绒毡层在解体时酸性磷本酶活性很高，两种不同的绒毡层退化过程相似，在全部发育过程中绒毡层内无淀粉粒。

TAPETUM DIMORPHISM AND ITS HISTOCHEMICAL STUDY IN SWEET PEPPER(CAPSICUM ANNUUM L.VAR.GROSSUM)

In sweet pepper,the portion of tapetum toward the interior of the anther comprising large cells is derived from cells of connective of anther whereas the remaining tapetum on the outside of the anther comprising comparatively small cells is derived from the parietal layer.Those cells of the former prosessing large vacuoles and large nuclei are stained we- aker than the cells of the latter by methyl green-pyronin and mercuric-bromophenol blue sta- ining.Large spherical grains which contain acid phosphatase appear in the vacuoles in both kinds of tapetum at spor0genesis stage.During meiosis of pollen mother cells,DNA,RNA and protein sysntheses increase in tapetum.The tapetum derived from connective accumu- lates more DNA than that derived from parietal layer.The activity of acid phosphatase be- comes higher in tapetum when it degenerates.The degeneration of two kinds of tapetum is similar.There are no starch grains in tapetum through its whole course of development.