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甘薯质体遗传方式的DNA指纹图谱分析
引用本文:方晓华,胡适宜,等.甘薯质体遗传方式的DNA指纹图谱分析[J].Acta Botanica Sinica,2003,45(1):73-75.
作者姓名:方晓华  胡适宜
作者单位:[1]中国科学院发育生物学研究所,北京100080 [2]北京大学生命科学学院,北京100871
基金项目:国家自然科学基金(39470046)~~
摘    要:用DNA指纹图谱分析了甘薯(Ipomoea batatasLam.)徐薯18和AB78-1品系及它们的正反交子代叶绿体DNA,结果显示子代叶绿体DNA指纹均与母本相同,而未发现与父本或双亲相同的指纹图谱,因此在该杂交组合中质体遗传方式为母系遗传,这个结论与先前根据细胞学研究所推测的甘薯质体遗传试不同。表明旋花科植物可能并不存在一个一致的父系或双亲质体传递模式。DNA指纹图谱分析用于质体遗传的研究尚未见报道,本文对其优越性进行了讨论。

关 键 词:正反交子代  甘薯  质体遗传方式  DNA指纹图谱分析

Plastid Inheritance in Sweet Potato as Revealed by DNA Restriction Fingerprinting
Authors:FANG Xiao-Hua  ZHANG Fang  WU Nai-Hu  HU Shi-Yi
Abstract:The inheritance of chloroplast DNA (cpDNA) in sweet potato (Iponwea batatas Lam.) was analyzed using DNA restriction fingerprinting. The cpDNA fingerprints of hybrids from reciprocal crosses between Xushu 18 and AB78-1 were found to be identical to those of their female parents, which reveals that cpDNA of sweet potato is maternally inherited in this intervarietal crossing. This maternal cpDNA transmission pattern does not accord with the putative one based on former cytological studies. The plastid inheritance in Convolvu-laceae has been briefly reviewed in this study, and the utility of DNA restriction fingerprinting analysis in the study of plastid inheritance is also discussed.
Keywords:Ipomoea batatas  plastid inheritance  DNA restriction fingerprinting  hybrids of reciprocal cross  Convolvulaceae
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