传粉导致的转基因白菜与其近缘种属材料间的基因流动 |
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引用本文: | 刘凡,赵泓,姚磊,张军杰,曹鸣庆.传粉导致的转基因白菜与其近缘种属材料间的基因流动[J].Acta Botanica Sinica,2003,45(6):681-687. |
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作者姓名: | 刘凡 赵泓 姚磊 张军杰 曹鸣庆 |
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作者单位: | [1]北京蔬菜研究中心,北京100089 [2]北京农业生物技术中心,北京100089 |
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基金项目: | 北京市科技合同项目 (95 44 13 5 0 0 ),北京市自然科学基金 (5 992 0 0 6)~~ |
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摘 要: | 从形态、染色体及分子水平上证实,转基因不结球白菜(Brassica campestris ssp.chinensis var.utilis Tsen et Lee)中编码除草剂Basta抗性的bar基因能在田间条件下,经自然传粉,以较高频率侵入芜菁(B.campestris ssp.rapifera)、结球白菜(B.campestris/sssp.pek/nens/s)和不结球白菜(B.campestrs ssp.ch/nens/s)的基因组中,也能少量侵入同属异种的甘蓝型油菜(B.napus)基因组中;在温室人工辅助授粉条件下,除在上述种中的基因漂移率提高外,bar基因尚能以一定频率侵入同属的黑芥(B.nigra)、埃塞俄比亚芥(B.car/nata)、芥菜(B.juncea)基因组中,但始终未能得到转基因白菜与结球甘蓝(B.oleracea)、萝卜(尺.sativus)的杂种。转基因白菜与十字花科的7种常见杂草经温室人工辅助授粉,也均未得到抗性杂种。
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关 键 词: | 转基因白菜 花粉传播 十字花科植物 基因入侵 基因流动 |
Gene Flow from Transgenic Chinese Cabbage (Brassica campestris ssp. chinensis var.utilis) to Related Cruciferae Species Through Outcrossing |
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Authors: | LIU Fan ZHAO Hong YAO Lei ZHANG Jun_Jie CAO Ming_Qing |
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Institution: | LIU Fan 1,ZHAO Hong 1,YAO Lei 1,ZHANG Jun_Jie 1 *,CAO Ming_Qing 2 |
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Abstract: | It was substantiated by morphological observation, chromosome and DNA identification that the bar gene, conferring the resistance to herbicide basta in transgenic Chinese cabbage ( Brassica campestris ssp. chinensis var. utilis Tsen et Lee), could intrude into the genomes of turnip ( B. campestris ssp. rapifera ), heading Chinese cabbage ( B. campestris ssp. pekinensis ) and other non_heading Chinese cabbage ( B. campestris ssp. chinensis ) in a relatively high frequency while into genome of B. napus at a low frequency via natural cross_pollination in open field. Herbicide resistant hybrids of B. nigra , B. carinata , and B. juncea could be obtained at certain frequency in the greenhouse by hand pollination. At no time did the resistant hybrid plants of transgenic Chinese cabbage with B. oleracea , R. sativus and seven wild weeds, widespread in China, gained in our experiments. |
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Keywords: | transgenic Chinese cabbage cross pollination Cruciferae species introgression |
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