喜树碱和10—羟基喜树碱受喜树生长发育调控的不同特点

为了揭示喜树碱（camptothecin,CPT)和10-羟基喜树碱（10-hydroxycamptothecin,HCPT)在喜树（Camptotheca acuminata Decaisne)体内代谢的生理调控机制及这两种类似物之间的关系和作用。运用高效液相色谱技术对种子形成，成熟，萌发和幼苗生长过程中喜树三和10-羟基喜树碱的代谢动态进行了全面的研究。结果表明，喜树碱相对稳定地存在于成熟的幼嫩的组织中；10-羟基喜树碱特异性地积累在乳熟期的种子，种芽的子叶，幼嫩的真叶等幼嫩组织中，随着组织的进一步发育和成熟，其含量快速减少，成熟组织中积累极少，并且这两种生物碱的代谢在时间和数量上都呈现出相互消长的特点，由此推断，这两种生物碱不同程度地受生长发育调控的特点可能与喜树的化学防御策略密切相关。并且，它们之间可以相互转化以充分利用有限的氮素资源。

Camptothecin and 10-Hydroxycamptothecin Accumulate Differentially Under Specific Developmental Control in Camptotheca acuminata

Camptotheca acuminata Decaisne is a tree unique to China, producing terpenoid indole alkaloid (TIA), camptothecin (CPT), and 10-hydroxycamptothecin (HCPT), which possesses strong anti-cancer bioactivity. The dynamics of HCPT and CPT during seed maturation, seed germination, postgerminative development and daily growth were measured by High Performance Liquid Chromatography (HPLC). This article provides some evidence for presenting the theory that each characteristic of HCPT and CPT accumulation is under developmental regulation and then attempt to elucidate the metabolic relationships between them. HCPT accumulates particularly in young tissues and organs and is relatively unstable, while the distribution of CPT is more ubiquitous and stable. Their different distribution properties suggest that HCPT and CPT are differentially regulated and play distinct roles during development.