小麦不同细胞质雄性不育系及其保持系萌动胚及幼芽可溶性蛋白等电聚焦电泳分析

用等电聚焦电泳分析的方法，测定了小麦（Ｔｒｉｔｉｃｕｍ ａｅｓｔｉｖｕｍ ）３ 种细胞质雄性不育类型（Ａ 型、Ｅ型、Ｔ型）及其相应同核保持系萌动胚及幼芽可溶性蛋白． 发现雄性可育系等电点（ｐＩ）为４．９０ 的蛋白质合成数量高于相应的不育系；ｐＩ为６．８５ 的蛋白质可能是Ｔ 型细胞质基因表达的结果；ｐＩ为７．６ 的蛋白质可能为津丰Ａ 不育系特有的区带．表明细胞质来源不同的不育类型，其萌动胚及幼芽可溶性蛋白等电聚焦电泳图谱差异明显，有可能作为鉴别它们的依据

ISOELECTROFOCUSING ELECTROPHORETIC ANALYSIS ON SOLUBLE PROTEIN OF GERMINATED EMBRYOS AND YOUNG SHOOTS FROM DIFFERENT CYTOPLASMIC MALE STERILE LINES AND THEIR MAINTAINERS IN WHEAT

The soluble proteins of germinated embryos and young shoots in Wheat were studied by means of isoelectrofocusing (IEF) electrophoresis. The materials used were three species of cytoplasmic male sterile (CMS) lines (Type E, T and A) and their maintainers. The protein quantity of pI 4 9 in male fertile materials is higher than the corresponding male sterile ones; the protein of pI 6 85 is probably the result of gene expression in Timopheevi cytoplasm; the protein of pI 7 6 is a typical band of Jinfeng male sterile line. Evident differences on soluble protein chromatograms of IEF electrophoresis were observed both in germinated embryos and young shoots which were from different kinds of CMS line, these differences could be used as indexes identifying various CMS lines.