| 一个小麦丝氨酸—苏氨酸蛋白激酶基因的克隆和分析 |
| |
| 引用本文： | 牛吉山,于玲,等.一个小麦丝氨酸—苏氨酸蛋白激酶基因的克隆和分析[J].Acta Botanica Sinica,2002,44(3):325-328. |
| |
| 作者姓名： | 牛吉山 于玲 |
| |
| 作者单位： | [1]国家小麦工程技术研究中心，郑州450002 [2]南京农业大学农业部作物细胞遗传重点实验室，南京210095 |
| |
| 基金项目： | 国家“8 6 3”高技术研究计划资助 (Z_17_0 4_0 1)，教育部高等学校重点开放实验室访问学者基金资助~~ |
| |
| 摘 要： | 用mRNA差异显示技术在含有抗白粉病基因Pm2 1的小麦 (TriticumaestivumL .)_簇毛麦 (Haynaldiavillosa)6VS/ 6AL易位系 92R137中分离与抗白粉病相关的基因 ,获得一个命名为TaPK1的全长cDNA克隆。序列分析表明 ,它与大豆 (Glycinemax (L .)Merr.)蛋白激酶基因GmPK6高度同源。经推测 ,TaPK1编码 416个氨基酸的多肽 ,属丝氨酸_苏氨酸蛋白激酶家族 ,并具酪氨酸激酶特性。TaPK1是从小麦中分离的新基因。
|
| 关 键 词： | 小麦 白粉病 蛋白激酶 |
Molecular Cloning and Characterization of a Serine/Threonine Protein Kinase Gene from Triticum aestivum |
| |
| Authors: | NIU Ji_Shan YU Ling MA Zheng_Qiang CHEN Pei_Du LIU Da_Jun |
| |
| Institution: | NIU Ji_Shan *,YU Ling,MA Zheng_Qiang,CHEN Pei_Du **,LIU Da_Jun |
| |
| Abstract: | To isolate genes related to resistance to Erysiphe graminis DC. ex Merat f. sp. tritici Em. Marchal in wheat (Triticum aestivum L.), differential display analysis was conducted for mRNA extracted from the seedlings of the wheat_Haynaldia villosa 6VS/6AL translocation line 92R137 that contains the powdery mildew resistance gene Pm21. A full_length cDNA named TaPK1 was isolated. BLAST analysis revealed that it was significantly homologous to Glycine max (L.) Merr. protein kinase (GmPK6) cDNA. TaPK1 encodes a 416 amino acid long polypeptide, which belongs to serine/threonine protein kinase family, also has tyrosine kinase specificity. TaPK1 is a novel protein kinase from wheat. |
| |
| Keywords: | wheat powdery mildew protein kinase |
| 本文献已被 维普 等数据库收录！ |
