| 五唇兰未受精胚珠离体培养及雌配子体发育的研究 |
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| 引用本文： | 伍成厚,梁承邺,叶秀麟.五唇兰未受精胚珠离体培养及雌配子体发育的研究[J].Acta Botanica Sinica,2004,46(7):839-845. |
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| 作者姓名： | 伍成厚 梁承邺 叶秀麟 |
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| 作者单位： | 中国科学院华南植物研究所 广州510650
(伍成厚，梁承邺)，中国科学院华南植物研究所 广州510650(叶秀麟) |
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| 基金项目： | 国家自然科学基金(30170061)，广东省重大科技专项(2003A2010401)。~~ |
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| 摘 要： | 五唇兰(DoritispulcherrimaLindl.)的胚珠属于倒生型,具薄珠心,两层珠被。胚囊发育类型为双孢子葱型,授粉后约45d形成七细胞八核的成熟胚囊。五唇兰未受精胚珠在离体培养初期对外源激素的依赖性很小,在没有外源激素的培养基上,大孢子母细胞也能经过减数分裂发育为二核胚囊。在培养后期,外源激素对胚囊发育的影响很大。在培养基无外源激素或仅含生长素或细胞分裂素时,雌配子体的发生过程不能顺利完成;在改良VW培养基上添加0.5mg/LBA和0.1mg/LNAA时,形成成熟胚囊。
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| 关 键 词： | 五唇兰 胚珠 胚囊 离体培养 胚胎学 |
In Vitro Megagametophyte Development via Unfertilized Ovule Culture in Doritis pulcherrima |
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| Authors: | WU Cheng-Hou LIANG Cheng-Ye YE Xiu-Lin |
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| Institution: | WU Cheng-Hou,LIANG Cheng-Ye,YE Xiu-Lin* |
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| Abstract: | The ovule of Doritis pulcherrima Lindl. is anatropous, tenuinucellate and bitegmic. The maturemegagametophyte is composed of seven cells with eight nuclei and its formation belongs to Allium type. Itsembryo sac matures about 45 d after pollination. The megasporogenesis in vitro by ovule culture can beinduced on all media whether with exogenous hormones or not 3 d after inoculation, but the latermegagametogenesis will stop on the medium without hormone or only with auxin or cytokinin. Matureembryo sac can form from megaspore mother cell on the modified VW medium supplemented with 0.5mg/L BA and 0.1 mg/L NAA. |
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| Keywords: | Doritis pulcherrima ovule embryo sac in vitro embryology |
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