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导入蜘蛛杀虫肽基因的烟草具有抗虫性
引用本文:蒋红,朱玉贤.导入蜘蛛杀虫肽基因的烟草具有抗虫性[J].Acta Botanica Sinica,1996,38(2):95-99.
作者姓名:蒋红  朱玉贤
作者单位:北京大学生命科学学院蛋白质工程及植物基因工程国家重点实验室!北京100871
摘    要:用带有杀虫肽基因的农杆菌(Agrobacterium tum efaciens)LBA4404 转化烟草(Nicotiana tabacum )叶片,共获得30 株抗卡那霉素的再生植株. 用这些再生植株对棉铃虫(Heliothisarm igera)进行毒力测定,有3 株转杀虫肽基因植株对棉铃虫有较强抗性. 与对照相比,这3 株转基因烟草的杀虫率可达30%~45% ,并能显著抑制昆虫蜕皮和生长发育,表现出明显的抗虫作用. 以这3 株为主进行了PCR 扩增及Northern blot实验,结果表明杀虫肽基因已插入到这3 株植株的基因组中并表达出有活性的杀虫肽

关 键 词:蜘蛛杀虫肽  烟草转化  抗虫性

INSECT RESISTANCE OF TRANSFORMED TOBACCO PLANTS WITH GENE OF THE SPIDER INSECTICIDAL PEPTIDE
Authors:Jiang Hong  Zhu Yu xian and Chen Zhang liang
Abstract:Tobacco (Nicotiana tabacum)leaves were transformed with Agrobacterium tumefaciens LBA4404 containing the insecticidal peptide gene.Thirty regenerated kanamycin resistant plants were obtained,among which three showed stronger toxicity to Heliothis armigera by feeding experiments.In comparision with feeding of the control plants,mortality of the insects fed on transgenic plants was significantly higher and the growth of the survived insects was remarkably retarded.Results of PCR Southern blot and Northern blot showed that insecticidal peptide gene has been transferred into the genome of these three plants and expressed efficiently to confer the insect resistance of the transgenic plants.
Keywords:Spider insecticidal peptide  Transformation of tobacco  Insect resistance
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