菊苣薄层培养花芽,营养芽分化中内源激素的动态变化

菊苣（Ｃｉｃｈｏｒｉｕｍ ｉｎｔｙｂｕｓＬ．）花梗薄层细胞培养于ＭＳ附加ＮＡＡ 和ＢＡ 或ＩＡＡ 和ＢＡ 的ＭＳ培养基上有花芽或营养芽分化． 花芽分化中内源ＩＡＡ、ＤＨＺ＋ ＤＨＺＲ、ｉＰＡ 含量明显增加，而Ｚ＋ ＺＲ变化不明显．营养芽分化中内源细胞分裂素含量增加明显，而ＩＡＡ 在培养前７ ｄ 含量下降，随后有所增加，在原基形成时含量达原初水平的２／３． 可见，花芽分化比营养芽分化所需内源ＩＡＡ／ＣＴＫ 比值要高

CHANGES OF ENDOGENOUS HORMONE CONTENTS DURING FLORAL BUD AND VEGETATIVE BUD DIF FERENTIATION IN THIN CELL LAYER CULTURE OF CICHORIUM INTYBUS L. EXPLANT

The sectioned thin cell layers (TCL) of flower stalk of Cichorium intybus L. were cultured in MS medium supplemented with NAA and BA or IAA and BA where floral and vegetative buds were developed from the explant. Endogenous IAA, DHZ DHZR, iPA increased significantly during the floral bud formation, while Z ZR remained changed. The levels of cytokinins, DHZ DHZR, iPA, and Z ZR all increased significantly during the vegetative bud formation, however IAA level was reduced during the first 7 days of culture and increased to two thirds of initial values on the day when the bud primordia were formed. The results suggested that the initiation of floral buds was associated with a high IAA/CTK ratio, whereas the induction of vegetative bud differentiation was related to a low IAA/CTK ratio.